CHARACTERIZATION OF THE ORAL AND ANAL MI CROBIOME OF MEN WHO HAVE SEX WITH MEN (MSM) AND TRANSGENDER WOMEN (TGW) IN ASSOCIATION WITH HIV INFECTION.

EZEQUIEL LACUNZA; MARIA E SALAS; ROMINA CANZONERI; VALERIA FINK; OMAR SUED; JULIÁN NAIPAUER; OMAR COSO; PEDRO CAHN; SION WILLIAMS; ENRIQUE A MESRI; MARTIN ABBA

Congreso; ANNUAL MEETING OF BIOSCIENCE SOCIETIES 2021; 2021

MSM and TGW are highly affected by HIV in Argentina. Aim to provide the first microbiome portrait of oral and anal mucosa from a cohort of HIV- and HIV+ MSM and TGW from Argentina. Methods Eighty samples of DNA derived from 40 oral swabs (OS) & 40 anal swabs (AS) were provided by 45 participants. Shotgun metagenomics sequencing was conducted with the Illumina NovaSeq 6000 System. NGS data analysis was performed using CosmosID, Humman3, and R/Bioconductor. Results In AS HPV types were among the most frequent virus, while Prevotella copri and Prevotella corporis were among the most abundant bacteria. OS were characterized by the prevalence of KSHV, EBV, HSV-2 and HHV-7, among others; while species of Haemophilus, Rothia, and Neisseria were the most frequent bacteria. Comparisons between HIV+ and HIV-, considering MSM and TGW, together or separately, indicated a distinctive set of differentially abundant taxa for each comparison (LogFC>1.5, p