DECREASED MIR-133A-3P AND MIR-133B EXPRESSION IN TUMORS FROM HIGH-FAT DIET FED MICE IMPACTS IN PROSTATE CANCER DEVELOPMENT

MASSILLO C; DUCA, RB; DALTON GN; LACUNZA E; DE SIERVI A

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

Recent evidence has revealed that high-fat diet (HFD) plays a role in prostate cancer (PCa) development and progression. Our hypothesis is that HFD alters miRNAs expression favoring prostate tumor growth. To assess this hypothesis, C57BL/6J male mice were chronically fed with HFD or control diet (CD). Murine TRAMP-C1 PCa cells were injected s.c on HFD and CD fed mice. After tumor growth, mice were sacrificed, tumors were collected and miRNAs isolated and analyzed by GeneChip® miRNA 4.0 Array (Affymetrix) microarrays. We identified 18 down- and 8 up-regulated miRNAs (FDR