Temporal and spatial expression of mucins MUC1 and MUC5AC during rat development.

FERRETTI; E LACUNZA; BARBEITO C; SEGAL EIRAS A; CROCE MV

Congreso; 5th International Meeting of the Latin American Society for Developmental Biology V; 2010

The transmembrane glycoprotein Muc1 is expressed at the apical surface of most adult epithelia while the Muc5ac is a a secreted mucin that polimerizes on the epithelia cell surfaces forming a mucus gel. In adult tissues Muc5AC is sinthesysed mainly by gastric and respiratory tissues. The objective of this research was to compare the pattern expression and tissue specific localization of Muc1 and Muc5ac during rat embrionic development.By IHC Muc1 expression was observed in several embryonic epithelial tissues being stomach, lung and kidney the first to show reaction on the 13th day of gestation. Muc5AC expression was observed in lung and gastric epithelia the intensity of reaction varied from low to strong: the esxpression was detected from D14 in Lung and from D17 in gastric epithelium, Interesitngly Muc 5AC was detected in the epidermis from D13 to D20. IHC results were validated by WB; by this technique, an increased expression of the mucin was observed which correlates with the development progress. Our results showed that Muc1 and Muc5AC expression depends of the time of development and also the tissue considered.