Engineering Pseudomonas for nitrogen fixation

SETTEN L; SOTO G; MOZZICAFREDDO M; CUCCIOLONI M; PAGANO E; DIAZ-PALEO A; AYUB ND

Mendoza

Congreso; SAIB; 2012

the beneficial Pf5 was genetically modified for nitrogen fixation, using the genes encoding the nitrogenase complex. This recombinant strain was able to grow in L médium without ammonium sulphate and displayed high nitrogenase activity. Furthermore, inoculatiom of Arabidopsis thaliana, Medicago sativa and  Tall fescue with the recombinant strain increased plant productivity more tan 100% under nitrogen-deficient conditions. Finally, we discussed the production of new recombinant inoculants.