Effect Of Low Levels Of Aflatoxin B1 On Performance, Biochemical Parameters And Aflatoxin B1 In Broilers Liver Tissues In Presence Of Monensin And Sodium Bentonite´S.

A. P. MAGNOLI; M. P. MONGE,

Jornada; - VI jornada científico- Técnicas de la facultad de agronomía y veterinaria r; 2013

Aflatoxins (AF) are a major problem in broiler production and are significant economic and public health burdens worldwide. A commercial sodium bentonite (Na-B) adsorbent was used to prevent the effect of AF [50 μg of aflatoxin B1 (AFB1)/kg of feed] in broiler productivity, biochemical parameters, macroscopic and microscopic liver changes, and AFB1 liver residues. The influence of Na-B (0.3%) and monensin (MON, 100 mg/kg), alone or in combination, was investigated in depth. The dietary treatments were as follows: treatment (T) 1: basal diet (B); T2: B + MON; T3: B + Na-B; T4: B + Na-B + MON; T5: B + AFB1; T6: B + AFB1 + Na-B + MON; T7: B + AFB1 + MON; T8: B + AFB1 + Na-B. Birds were fed dietary treatments for 28 d (d 18 to 46). No significant differences (P < 0.05) were observed among treatments with respect to broiler performance, biochemical parameters, or relative liver weights. With the exception of T8, all livers showed histopathological alterations, with accumulation of fat vacuoles. The normal appearance of livers from T8 showed the protective effect of Na-B against aflatoxicosis. The residual AFB1 levels in livers from T5 to T8 ranged from 0.2 to 1.0 ng/g and were higher in livers from T6 (P < 0.05). Results of this study indicate a competition between AFB1 and MON for adsorption sites on Na-B when feed contains low levels of the toxin, indicating a nonselective adsorption capacity of this particular Na-B. In addition, significant levels of AFB1 in livers indicate that this determination is an important technique not only for diagnosis of aflatoxicosis in broilers, but also for quality control of avian products.