In vitro effect of reduced flubendazole metabolite against echinococcus granulosus protoscoleces

ELISSONDO, C; CEBALLOS, L; ALVAREZ, L; SANCHEZ BRUNI, S; LANUSSE, C; DENEGRI, G

. Rosario. Argentina

Congreso; XLI Reunion Anual de la Asociación Argentina de Farmacología Experimantal; 2009

Flubendazole (FLBZ) showed efficacy against protoescólices (PSC) and cysts of Echinococcus granulosus in vivo and in vitro. After de oral administration to mice, FLBZ concentrations within the cysts were significantly higher than those measured for its reduced (R-FLBZ) and hydrolyzed metabolites. The aim of this work was to point out the in vitro effect of the R-FLBZ against E. granulosus PSC. PSC were incubated with 11 nm of FLBZ, R-FLBZ, ABZ or ABZSO. PSC incubated with culture medium containing methanol were used as controls. Vitality was assessed every 6 days using the methylene blue exclusion technique and samples were taken for electron microscopy. As previously shown, the greater effect was produced by FLBZ. R-FLBZ showed a marked effect producing ultrastructural alterations after 6 days post-incubation and reducing the vitality to approximately 27% after 54 days. The outcomes obtained demonstrated the in vitro efficacy of the R-FLBZ against PCS.