Glyphosate utilization by bacteria isolated from a South-of-Santa Fe-VerticArgiudoll

CARNÉ. IGNACIO; VIASSOLO, RODRIGO; ZABALOY, MARÍA CELINA; GOMEZ, ELENA V

Rosario

Congreso; XIV Congreso y XXXII Reunión Anual de la Sociedad de Biología de Rosario; 2013

Sociedad de Biología de Rosario

Glyphosate (gf) (N-phosphonomethyl glycine) is the active ingredient of many herbicides used in weed control nowadays, which can be toxic for non-target organisms. Bacterial bioremediation is an alternative to eliminate this xenobiotic from environment. Bacteria strains able to use gf were isolated from soils from Zavalla (Sta.Fe). Bacteria were obtained by enrichment in a mineral salt medium (MSM) supplemented with yeast extract (y.e.) and gf (technical grade, 1mM) as phosphorous (P) source. Experiments were carried out in 3 replicates and incubation temperature at 30ºC. One isolated strain,identified as Rhodococcus wratislaviensis strain NCIBM 13082 (accession NR-026524.1, GenBank ) was able to use gf (1 mM) as P source in MSM without y.e. when glucose was added as carbon and energy source. Time course of growth (optical density at 660 nm) at different inputs concentrations of gf (1,5; 3.0 and 10,0 mM) showed significant difference (p< 0,05) at 10 mM. This strain could potentially be used as a model microorganism to evaluate glyphosate environmental decontamination or toxicological tests.