Identification of potential biomarkers of protection in bovine tuberculosis

BLANCO, FEDERICO CARLOS; SORIA M.; BIANCO, MARÍA VERÓNICA; GARCIA, E; KLEPP, LAURA INÉS; BIGI, FABIANA

Canela, RS

Congreso; VII Meeting of the SLAMTB; 2014

Sociedad Latinoamericana de Tuberculosis y otras Micobacteriosis

Background and objectives: The purpose of the present study was to identify genes with differential expression in peripheral blood mononuclear cells (PBMC) from vaccinated protected and unprotected cattle against challenge with a pathogenic strain of M. bovis. Methods: In a bovine vaccine trial we evaluated the protection conferred by two different vaccine candidates. Vaccinated cattle were divided into three groups according to their pathology score, vaccinated/protected (n=4), vaccinated/unprotected (n=4) and vaccinated with intermediate pathology score (n=2). Total ARN was obtained from PBMC samples stimulated with purified protein derivative of M. bovis (PPDB) at 60 days post vaccination. Using Affymetrix GeneChip® Bovine Genome Array we analized the transcriptomic profile of the ARN samples searching for differential gene expression among groups. Results: In a preliminary analysis of the data we observed 55 genes differentially expressed (p