THE REPARATION CAPACITY AT LOW DOSES OF RADIATION

ROSEMBLIT C; PISAREV M; IBAÑEZ IL; CREMASCHI GA; JUVENAL GJ; PERONA M; GRISSI C; DURAN H

Virtual

Congreso; Reunión Anual de Sociedades de Biociencias SAIC, SAI, SAFIS; 2020

Sociedad Argentina de Investigaciones Clínicas

Introduction: Histone deacetylase (HDAC) inhibitors have emerged recently as promising anticancer agents. The antitumor activity of HDAC inhibitors has been linked to their ability to induce gene expression through acetylation of histone and nonhistone proteins. Anaplastic thyroid cancer (ATC) is a rare and aggressive malignancy. Radiotherapy (RT) is one of the main modalities of treatment for ATC. In most patients, surgical resection is not possible. Therefore, RT either as altered fractionation or in combination with chemotherapy has an important role in achieving local control. Objectives: The objective was to study the radiosensitizing effect of valproic acid at different radiation doses in an anaplastic thyroid cancer cell line (8505c). Methods: Cells were incubated with 1 mM VA and irradiated with a source of gamma rays at different doses. Radiation response was analyzed by clonogenic assay. Cell cycle and cell death were measured 24 and 48 h after irradiation at 2 and 5 Gy. DNA damage was evaluated 30 min and 24 h after irradiation at 2 and 5 Gy. Ku80 expression was assessed by Western Blot. Results: A radiosensitizing effect was observed with a reduction of survival fraction at 2 Gy from 27.7±0.04 to 20.0±0.02 in the treated cells (p