Radiosensitization of human melanoma cells by superparamagnetic iron oxide nanoparticles

M. PERONA; M. F. DEL GROSSO; M. TAVERNA PORRO; J. SACANELL; H. DURÁN; C. GRISSI; S. MORENO; I. IBAÑEZ

Otro; Reunión Conjunta de Sociedades de Biociencias (SAIC/SAI/AAFE/NANOMED-Ar); 2021

Melanoma is the deadliest form of skin cancer, highly metastatic andresistant to therapies. Superparamagnetic iron oxide nanoparticles(SPIONs) have shown great potential for diagnosis and therapy. Theaim of this study was to evaluate the radiosensitizing effect of SPIONsin A375 human melanoma cells.SPIONs were synthesized and stabilized by methyl-poly(ethyleneglycol). After that, it was physicochemically characterized by transmissionelectron microscopy, X-ray diffraction, magnetometry andtested in vitro. Superparamagnetic behavior and low dispersionin shape and sizes (8-17 nm) were obtained. No cytotoxicity wasfound in A375 cells exposed up to 250 μg/ml for 24 h. Dichloro-dihydro-fluorescein diacetate assay revealed higher reactive oxygenspecies levels in treated cells (p