UE-INN 27105
UNIDAD EJECUTORA INSTITUTO DE NANOCIENCIA Y NANOTECNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
High-throughput meta-analysis and validation of differentially expressed genes as potential biomarkers of ionizing radiation-response
Autor/es:
NEGRIN, LARA; BIOLATTI, VANESA; IBAÑEZ, IRENE L.; BIOLATTI, VANESA; IBAÑEZ, IRENE L.; BELLORA, NICOLÁS; BELLORA, NICOLÁS; NEGRIN, LARA
Revista:
RADIOTHERAPY AND ONCOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Año: 2020
ISSN:
0167-8140
Resumen:
Background and purposeThe high-throughput analysis of gene expression in ionizing radiation (IR)-exposed human peripheral white blood cells (WBC) has emerged as a novel method for biodosimetry markers detection. We aimed to detect IR-exposure differential expressed genes (DEGs) as potential predictive biomarkers for biodosimetry and radioinduced-response.Materials and methodsWe performed a meta-analysis of raw data from public microarrays of ex vivo low linear energy transfer-irradiated human peripheral WBC. Functional enrichment and transcription factors (TF) detection from resulting DEGs were assessed. Six selected DEGs among studies were validated by qRT-PCR on mRNA from human peripheral blood samples from nine healthy human donors 24 h after ex vivo X-rays-irradiation.ResultsWe identified 275 DEGs after IR-exposure (parameters: |lfc|≥0.7, q value
