MODULATION OF PEPTIDOGLYCAN RECOGNITION PROTEINS-MEDIATED PORCINE INTESTINAL INNATE IMMUNITY BY PARAIMMUNOBIOTIC BIFIDOBACTERIA

AMINUL, MD ISLAM; HIKARU IIDA; MASANORI TOHNO; RYUSUKE OHGI; LEONARDO ALBARRACÍN; WAKAKO IKEDA-OHTSUBO; AYAKO MIYAZAKI; HIROHIDE UENISHI; SUDA, Y.; NORIYUKI IWABUCHI; JIN-ZHONG XIAO; JULIO VILLENA; HARUKI KITAZAWA

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Peptidoglycan recognition proteins (PGLYRPs) are able to induce innate immune responses through their binding to pepti¬doglycan (PGN) or by interacting with other pattern recognition receptors (PRR)-ligands in the intestinal mucosa. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however their functions in livestock has been less explored. In this study, the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen presenting cells (APCs) were characterized. In addition, the modulation of PGLYRPs activation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory bifidobacteria (paraimmunobiotic bifidobacteria) was studied. Immunofluorescence and qPCR analysis showed that PGLYRP-1, -2, -3 and -4 are expressed in PIE cells and APCs from Peyer´s patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2 (p