INVESTIGADORES
ROCA Valeria Ines
congresos y reuniones científicas
Título:
Macrophages resident in NOD mice salivary glands present different basal activity and a lower anti-inflammatory effect of VIP
Autor/es:
MARIO CALAFAT; LUCIANA LAROCCA; VALERIA ROCA; NICOLAS PREGI; ALCIRA NESSE; NELSON DUSETTI; CLAUDIA PEREZ LEIROS
Lugar:
Madrid, España
Reunión:
Congreso; II Congreso Iberoamericano de Neuroimunomodulación; 2007
Resumen:
The non obese diabetic (NOD) mouse is a suitable experimental
model to study autoimmune sialadenitis resembling Sjögrens syndrome. Vasoactive intestinal peptide (VIP) is an immuno-neuropeptide mediating
secretory and anti-inflammatory responses. In previous work we showed an
increased expression of TP53INP1 in both 8 and 16 weeks-old female NOD mice.
However, only at 16 weeks NOD mice showed apoptosis associated to a higher
expression of bax. TNF alfa is known to induce apoptosis in certain tissues.
Our goal was to study the role of resident macrophages of salivary glands from
female NOD mice on the apoptosis of acinar cells and the possible modulation by
VIP. We showed an increased TNF-alfa-induced apoptosis on the acini of NOD and
BALB/c mice associated to an increased expression of TP53INP1. Resident
macrophages of NOD salivary glands presented a different basal activity compared
to BALB/c, characterized by increased production of nitrites and TNF-alfa.
Although VIP inhibited LPS-stimulated TNF-alfa production in BALB/c mice, this
effect was not seen in NOD mice.