Gene homologues of Pseudomonas aeruginosa phosphorylcholine phosphatase in others species of Pseudomonas

BEASSONI, PR; MASSIMELLI, MJ; FORRELLAD, MA; GARRIDO, MN; DOMENECH, CE; LISA, AT

Villa General Belgrano, Córdoba

Congreso; XIV Reunión Anual, Sociedad Argentina de Córdoba (SBC); 2003

Sociedad de Biología de Córdoba

Phosphorylcholine phosphatase (PCP) and phospholipase C (PLC) are produced by Pseudomonas aeruginosa grown with choline, betaine or dimethylglycine. Choline and both enzymes have been involved in the bacteria pathogenesis. Different tissue membranes of the host organism are degraded as follow: PLC hydrolyses phosphatidylcholine to DAG and phosphorylcholine. This last compound, produces choline and Pi by PCP action. Recently, the responsible gene of PCP activity in P. aeruginosa, PA5292, was identified. The aim of this work was to know if the proposal mechanism for P. aeruginosa was only specific for this bacterium. PA5292 gene was amplified by PCR and used as a probe in Southern blot experiments. Homology was found in the plant pathogenic species: P. cichorii, P. syringae, P. agarici, P. tolaasii, P. aspleni and in the non-pathogenic species P. fluorescens, P. putida, P. aureofaciens and P. fragi. In these bacteria it was also detected PCP activity but not PLC activity. Gene homologues of the P. aeruginosa pcPwere identified by bioinformatic analysis. They are, the genes PP5130, Pflu3115, Psyr3119, PSPTO0436 of P. putida, P. fluorescens, P. syringae pv syringae and pv. tomato, respectively. The PCP presence and the absence of PLC homologue gene in the above mentioned bacteria, indicate that they do not share a similar infection mechanism with P.aeruginosa.