A Glance on Pseudomonas aeruginosa Phosphorylcholine Phosphatase, an enzyme whose synthesis depends on the presence of choline in its environment.

LISA, AT; BEASSONI, PR; MASSIMELLI, MJ; OTERO, LH; DOMENECH, CE

Communicating Current Research and Educational Topics and Trends in Applied Microbiology

FORMATEX (Editor: A. Méndez-Vilas)

Lugar: Baadajoz, España; Año: 2007; p. 255 - 262

This paper reports the discovery, plus kinetic, biochemical, biophysical and molecular characteristics of a P. aeruginosa phosphorylcholine phosphatase (PchP) whose synthesis depends on choline and derivatives in the culture medium. PchP is the product of the PA5292 gene in the P. aeruginosa PAO1 genome and it is principally transcribed as a monocistronic 1 Kb mRNA. PChP belongs to the haloacid dehalogenases hydrolase superfamily which contains totally three conserved motifs. In mature PChP, motifs I, II and III are the aminoacyl residues 31DMDNT35, 166S and K242/261GDTPDSD267, respectively. After site directed mutagenesis on these motifs, the more important residues for catalysis were identified. PchP is involved in the pathogenesis of P. aeruginosa through the coordinated and sequential action of hemolytic phospholipase C and PchP on phosphatidylcholine or sphyngomyelin and phosphorylcholine, respectively