Electroporation of Postimplantation Mouse Embryos In Utero

HUANG, CHENG-CHIU; CARCAGNO, ABEL

Cold Spring Harbor Protocols

Cold Spring Harbor Laboratory Press

Lugar: Long Island, New York; Año: 2018 vol. 2018 p. 99 - 102

1940-3402

Gene transfer by electroporation is possible in mouse fetuses within the uterus. As described in this protocol, the pregnant female is anesthetized, the abdominal cavity is opened, and the uterus with the fetuses is exteriorized. A solution of plasmid DNA is injected through the uterine wall directly into the fetus, typically into a cavity like the brain ventricle, guided by fiber optic illumination. Electrodes are positioned on the uterus around the region of the fetus that was injected, and electrical pulses are delivered. The uterus is returned to the abdominal cavity, the body wall is sutured closed, and the female is allowed to recover. The manipulated fetuses can then be collected and analyzed at various times after the electroporation. This method allows experimental access to later-stage developing mouse embryos.