Metagenomic analysis of begomovirus infecting pepper in the northeast of Argentina

BORNACINI VA; VAGHI MEDINA CG; FLORES CR; AMADIO AF; DUCASSE DA; LÓPEZ LAMBERTINI PM

New Delhi

Simposio; 8th International Geminivirus Symposium and 6th International ssDNA comparative virology Workshop; 2016

Horticultural crops are threatened by the emergence of new begomoviruses in Latin America. One of the main production areas where begomoviruses cause economic losses is the northeast of Argentina (NOA). In this region, pepper is an important crop mostly grown under greenhouse condition. Begomoviruses incidence is increasing probably due to the dissemination of its vector (Bemisia tabaci). The genetic diversity of begomoviruses infecting pepper has not been determined in Argentine and the aim of this study was to establish that diversity by metagenomic analysisin crops of NOA region. We analyzed by Multiplex-PCR 101 symptomatic peppers plants sampled on three locations named Pichanal, Oran, and Yuto during the period 2005?2014. From 52 begomovirus positive samples, 21 were selected to be amplified by RCA and gel purified. These 21 samples were sequenced using Illumina Hiseq1500 system (INDEAR). Bioinformatics analysis of the sequences resulted in the specific identification of Soybean blistering mosaic virus, Tomato yellow spot virus, Tomato yellow vein streak virus, and Tomato dwarf leaf virus. Soybean blistering mosaic virus, Tomato yellow vein streak virus and Tomato dwarf leaf virus were also found infecting tomato in the same years and production area. Besides, Soybean blistering mosaic virus was identified in soybean and bean crops while Tomato yellow spot virus was reported in soybean, bean and chia crops in Argentine showing the great adaptation of these viruses to different hosts.