Regulation of T cell response in the course of Clostridioides difficile infection.

ESPAÑOL LA; BARBERO AM; MORRO LS; VILLAFAÑE G; MACHAIN M; HERNÁNDEZ DEL PINO RE; PASQUINELLI V

BUENOS AIRES (VIRTUAL)

Congreso; Reunión Anual de Sociedades de Biociencias 2020, SAIC- SAI- SAFIS; 2020

SAI- SAIC- SAFIS

Clostridioides difficile infection (CDI) is the leading cause of antibiotic-associated diarrhoea. The innate immune response during CDI has been extensively explored, however, the phenotype and functions of T cells during CDI is only beginning to be understood. To characterize the adaptive immunity during CDI, C57/BL6 mice were treated with a cocktail of antibiotics and then infected with 105 spores of BI/NAP1/027 C. difficile strain. The expression of the co- stimulatory molecules SLAM and ICOS was evaluated at 5 and 8 days post-infection (pi) in lamina propria cells (LPMCs) and in mesenteric lymph nodes (MLNs) lymphocytes stimulated with/without PMA/Inomycin for 4h. Infected mice showed typical CDI symptoms (diarrhea, hunched posture, and weight loss (p