Clostridium difficile infection: Murine model development and immunological characterization

HERNÁNDEZ DEL PINO RE; CAJIAO MF; BARBERO AM; CELANO J; ESTERMANN MA; MACHAIN M; PASQUINELLI V

Mar del Plata

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS). LXIII Reunión Anual SAIC, LXVI Reunión Anual SAI, Reunión Anual SAFIS.; 2018

Clostridium difficile-associated disease is caused by a gram-positive, anaerobic, spore-forming bacterium Clostridium difficile (C.difficile) and is the most common cause of antibiotic associated diarrhea. BI/NAP1/027, an emerging strain of C. difficile, has dramatically changed the epidemiology of C. difficile infection (CDI) withoutbreaks of severe disease in North America and Europe. Antibiotics treatment is the standard method of therapy but also the majorcause of susceptibility to CDI. Therefore, identification of key effector molecules of host immune response against CDI may providenovel immunotherapies.After induction of dysbiosis, mice were infected with spores from thehypervirulent BI/NAP1/027 strain. Post-infected mice evidenced typical CDI symptoms such as diarrhea, hunched posture, and weightloss (p