The negative feedback mechanism between SLPI and IFN-g is impaired in tuberculosis patients

TATEOSIAN NL; PASQUINELLI V; HERNÁNDEZ DEL PINO RE; GARCÍA VE; CHULUYAN HE

Boston

Congreso; 99th Annual Meeting of the American Association of Immunologists; 2012

American Association of Immunologists (AAI)

Mycobacterium tuberculosis increases IFNγ secretion which plays an important role in granuloma formation and clearance of it. Also, the exposure of macrophages to M. tuberculosis increases secretory leukocyte protease inhibitor (SLPI), a serine protease inhibitor with antimycobacterial activity. Therefore, the aim of the present study was to determine the role of a putative interaction between IFNγ and SLPI in PBMC derived from healthy donors (HD) and tuberculosis patients (TB). Peripheral blood mononuclear cells (PBMC) from TB and HD were treated with Mtb antigen and IFNγ and SLPI was determined by ELISA. Our results showed that the stimulation with Mtb of PBMC from HD, it decreased SLPI (p