PERSONAL DE APOYO
MASCIARELLI Oscar Alberto
artículos
Título:
POTENTIAL BIOLOGICAL CONTROL PSEUDOMONAS sp. PCI2 AGAINST DAMPING-OFF OF TOMATO CAUSED BY SCLEROTIUM ROLFSII
Autor/es:
N.A. PASTOR, M.M. REYNOSO, M.L. TONELLI, O. MASCIARELLI, S.B. ROSAS AND M. ROVERA
Revista:
JOURNAL OF PLANT PATHOLOGY
Editorial:
EDIZIONI ETS
Referencias:
Lugar: Pisa; Año: 2010 p. 737 - 745
ISSN:
1125-4653
Resumen:
Fluorescent Pseudomonas spp. isolated from the
roots of healthy tomato plants were screened for their
antagonistic activities against Sclerotium rolfsii, Alternaria
alternata and Fusarium solani, three phytopathogenic
fungi of tomato and pepper. They were tested for phosphate
solubilization ability and production of
siderophores, hydrolytic enzymes, indole 3-acetic acid
and hydrogen cyanid. The isolates were also characterized
based on biochemical (API 20NE test) and genotypic
(ERIC-PCR fingerprinting) features. A Pseudomonas
sp. strain denoted PCI2 was chosen as a potential
candidate for controlling tomato damping-off
caused by Sclerotium rolfsii. PCI2 was identified at the
genus level with a 16S rDNA partial sequence analysis
and its phylogenetic relationship with previously characterized
Pseudomonas species was determined. PCI2
clustered with the P. putida species. Growth chamber
studies resulted in statistically significant increases in
plant stand (29%) as well as in root dry weight (58%).
PCI2 was able to establish itself and survive in tomato
rhizosphere after 40 days, following planting of bacterized
seeds. PCI2 is a potential biological control agent
that may contribute to the protection of tomato plants
against damping-off caused by S. rolfsii.