Acute Fe-dextran treatment and redox balance in rat whole brain and cortex

PILONI NE; ROBELLO E; PUNTARULO S

Congreso; Reunión Anual de Biociencias: SAIC, SAFE, SAB, SAP, NANOMEDar, AACyTAL, HSC; 2019

ACUTE FE-DEXTRAN TREATMENT AND REDOX BALANCE IN RAT WHOLE BRAIN AND CORTEX1,2N. E. Piloni; 1,2E. Robello and 1,2S. Puntarulo(1)Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Fisicoquímica. Buenos Aires, Argentina.(2)CONICET-Universidad de Buenos Aires. Instituto de Bioquímica y Medicina Molecular (IBIMOL). Buenos Aires, Argentina.An acute Fe-dextran treatment produced oxidative stress in rat brain that lead to the translocation of Nrf2 to the cell nucleus, producing the activation of genes involved in the glutathione metabolism in the cellular environment. Previous reports have shown that the acute Fe overload produced by a single injection of Fe-dextran resulted in a significant decrease in total thiol and glutathione content in rat cortex area after 6 and 8 h post injection (pi). In the whole brain, enzymatic activities of glutathione-S-transferase (GST) and glutathione peroxidase (GPx), and total thiol content were increased as compared to control tissues at 6 or 8 h pi, respectively. The aim of this study was to determine the effect of acute Fe overload on glutathione-dependent enzymatic metabolism in cortex rat brain. A single dose of 500 mg Fe-dextran/kg body weight was administrated intraperitoneally to male Sprague Dawley rats. Total brain samples or cortex area were obtained from control and treated animals after 6 or 8 h pi. Glutathione reductase (GR) was determined spectrophotometrically. Reduced glutathione (GSH), oxidized glutathione (GSSG) and malondialdehyde (MDA) content were determined by reverse phase HPLC. MDA content showed a significant increased (p