Distribution of Iron in Plasma in Endotoxemic Rats.

GALLEANO M; ROBELLO E; PUNTARULO S

Montevideo, Uruguay

Congreso; Free Radicals in Montevideo 2007. V Meetin of SFRBM- South American Group. V International Conference on Peroxynitrite and Reactive Nitrogen Species; 2007

The aim of this work was to study the role of nitric oxide (NO) on Fe distribution in plasma employing a model of endotoxemia. Female Sprague Dawley rats were administered with either lipopolysaccharide from Escherichia coli (serotype 0127:B8) (ip 4 mg/kg) (LPS), saline solution (C), L-NAME (ip 50 mg/kg) (L-NAME), or L-NAME simultaneously with LPS (L-NAME+LPS), and blood samples were obtained after 6h. NO production was followed by measuring NO-Hb in blood by EPR at 77 K. LPS administration increased NO-Hb detection by 10 times as compared to C group. Both, the Fe content (39±3 μM vs. 14±1 μM, p