Neural Precursor Cells Following gamma Irradiation is Early Modulated by Free

ROBELLO E; DUBNER D; MICHELIN S; PÉREZ MR; PUNTARULO S; GISONE P

Proceedings of the XII Biennial Meeting of the Society for Free Radical

Medimond SRL

Año: 2004; p. 299 - 303

Radiation-induced nitric oxide (NO) generation in cortical precursor cultures was highly increased within 1 h post-irradiation (pi) and free radical production was observed after 2 h pi. However, when NO production was inhibited by L-NAME, not only a higher radiation-induced luminol-dependent chemiluminiscense (CL) but also an enhanced radiation- induced apoptosis (at 24 h pi) was observed. Moreover, if SOD was added to L-NAME treated cells, a decline in CL occurred within 2 h pi. Inhibition of caspase-3 activity produced a decrease in CL after 2 h pi. These data indicated that NO could act as an antioxidant and neuroprotective factor in an early period immediately after irradiation in vitro; besides, it is suggested that caspase-3 activity is closely related to a late free radical generation.