INVESTIGADORES
VALDEZ Susana Ruth
congresos y reuniones científicas
Título:
Effects of hypo- and hyper-thyroidism on neuropeptide glutamic acid-isoleucine (NEI) in brain regions related to reproduction
Autor/es:
AYALA CAROLINA; SOAJE MARTA; VALDEZ SUSANA R; CARREÑO NORMA B; JAHN GRACIELA A; CELIS MARÍA ESTER
Lugar:
Córdoba
Reunión:
Congreso; Sociedad Argentina de Neuroquímica (SAN) y Workshop Argentina en Neurociencias; 2009
Institución organizadora:
Sociedad Argentina de Neuroquímica (SAN)
Resumen:
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NEI is a peptide derived from ppMCH that is capable of regulating the release
of TSH and LH.
Objective. To analyze whether hypo- or hyperthyroidism can modify the concentration
of NEI at different stages of the rat estral cycle, late pregnancy (day 19: G19
and 21: G21) and early lactation (postpartum day 2: L2), in brain areas related
to reproduction.
Materials and Methods. Female Wistar rats weighing between 180 and 250 g were divided into
control (C) and treated (T) groups. Hypo- and hyperthyroidism were induced with
PTU and T4, respectively. The studied brain areas were OVLT + AVPV (vascular
organ of the lamina terminalis + anteroventral
periventricular nucleus), POA (preoptic area), HLA (lateral hypothalamic
area), ZI (zona incerta), PeN (periventricular nucleus), ME + Arc (median
eminence + arcuate nucleus) and PP (posterior pituitary). NEI concentrations in
samples were assessed by RIA.
Results. Cycling females. OVLT +AVPV: the differences were observed in T, with the highest values
found in T4 P12. POA (area related to sexual behaviour): the lowest values were
for PTU and T4 P19h. HLA, higher concentration of NEI in C E12 that decreased
in T. ZI: the pattern between C and PTU was very similar with lower values in
the latter; the peptide was hardly modified in T4. No differences in C and T
were observed for PeN, and were minimal in ME +Arc. PP: there was a significant
increase in T E12h.
Pregnant females. A significant increase of NEI was observed in pregnant rats: in OVLT +
AVPV were found in T4 G19 compared to PTU, in T4 G21 compared to C and PTU, and
in C L2 compared to all other groups; in POA, in PTU G21 compared to C and T4; the
content of NEI in HLA for C L2 was greater than in T; increases in ZI were
observed in C and T4 G19 compared to PTU, and in C L2 compared to T; PeN showed
increases in T G21 compared to C; increases in ME + Arc were found in C L2
compared to PTU and T4 L2; and in PP, there were increases in T4 G21 compared
to C and PTU.
Conclusions. The content of NEI in different brain regions related to reproduction
is modified by hypo- or hyperthyroidism in cycling, pregnant and early
lactation females. The results found in POA in T probably reflect the changes
in copulatory female behaviour.
