Prenatal D-Amphetamine exposure influences prolactin synthesis and secretion in response to stress and estrogen in adulthood

SANTONJA FLORENCIA ELEONOTA; PIETROBÓN ELISA O; NEIRA FLAVIA J; JAHN GRACIELA A; VALDEZ, SUSANA RUTH; BREGONZIO CLAUDIA

Mar del Plata

Congreso; Sociedad Argentina de Investigación Clínica; 2019

SAIC

Abstract ID: 0676The dopaminergicsystem, a main regulator of prolactin (PRL) secretion, is closely involved in theresponses to stress and amphetamine. The ovarian steroids modulate prolactinsecretion and several aspects of stress responses. Previously, we found thatprenatal exposure to amphetamine (PEA) induced changes in pituitary mRNA D2Rexpression during adulthood. Ourpresent aim was to study PEA effects and the influence of estrogen (E2) on pituitaryD2R expression (protein) and their correlation with pituitary PRL content (mRNAand protein levels) in adult OVX rats in response to stress. Moreover, we alsoexplore the role of E2 in response to stress on the expression of de pTH-Ser 40in the medium basal hypothalamus(MBH) of OVX adult rats in PEA animals. Forthese purposes, female Wistar rats were treated daily with D-amphetamine2,5mg/kg i.p/saline during days 15 to 21 of pregnancy. Their offspring were OVXat day 60; 15 days later treated with estrogen/oil (E2; 2 x 5ug/rat/24 h) and exposedto immobilization stress during 30 min. Blood and tissue samples were collectedfor corticosterone by RIA and pituitary D2R and PRL content by real time PCRand Western blots (WB) determinations. pTHSer-40 expression was determined by WBin MBH extracts. Data were analyzed using two-way ANOVA and t-Student´s test. Results:pituitary D2R expression (protein) was diminished only in PEA OVX+E2 rats (p<0.05)and no effects of stress were observed. E2 increased PRL mRNA levels in controland PEA OVX rats p<0.05) and prevented the stress effect. Stress diminished PRLpituitary content (protein) in control rats with E2 and the PRL protein in PEArats independent of E2 (p<0.05). Moreover, stress decreased p-TH in MBH PEA OVX+E2rats (p<0.05). Thus, prenatal treatment with D-amphetamine sensitizes thehypothalamus-pituitary system affecting PRL synthesis and secretion in responseto stress and E2.&amp;amp;amp;lt;!-- /* Font Definitions */@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073786111 1 0 415 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;margin-left:0cm;line-height:115%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:Calibri;mso-bidi-font-family:"Times New Roman";mso-ansi-language:ES-AR;}span.tlid-translation{mso-style-name:tlid-translation;mso-style-unhide:no;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;mso-hansi-font-family:Calibri;}@page WordSection1{size:612.0pt 792.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&amp;amp;amp;gt;