“Effect of tumor necrosis factor receptor p55 deficiency on the severity of post-Yersinia reactive arthritis”

CARNELUTTI D; CASTRO D; IANIZZOTTO G; VALDEZ S; GUZMÁN A; DI GENARO

CórdobA

Congreso; VII CONGRESO LATINOAMERICANO DE INMUNOLOGIA (ALAI); 2005

The objetive of the present work was to study the rol of tumor necrosis factor receptor (TNFR) p55 in the Yersinia-associated arthritis by the use of a murine model of Yersinia enterocolitica infection. TNFRp55 deficient (TNFR p55-/-) and C57BL/6 mice were orally infected with 1x107 colony forming units (CFU) of Yersinia enterocolitica serotype O:3. Mice were daily observed and arthritis development was monitored by clinical score, joint assessment and x-ray. Mice were sacrificed at 3, 39 and 57 days post-infection (p.i.) for examination of histopathology of joints and bacterial clearance. There was a 42% mortality rate in TNFR p55-/- compared with 0% in controls. Bacteriological examination on day 57 showed completely Yersinia elimination in the spleen, mesenteric lymph nodes and joint. TNFR p55-/- mice developed arthritis at day 14 with severe inflammation and tarsus deformation on day 55 p.i. Erosion of cartilage and bone was observed in knockout mice on day 39 p.i. The results indicate that TNF signaling through TNFR p55 has a protective role in arthritis induced by oral Yersinia enterocolitica infection.