LEUKOCYTES INFILTRATION CHAPERONES HYPERTHYROIDISM-INDUCED INCREASE OF FETAL GROWTH, PLACENTAL CHANGES, AND IMPAIRED OFFSPRING DEVELOPMENT 

SÁNCHEZ MARÍA BELÉN; MORENO-SOSA MARÍA TAMARA; MICHEL, MARÍA CECILIA; PIETROBON, ELISA OLIVIA; SOAJE, MARTA; JAHN, GRACIELA A; VALDEZ SUSANA R; MACKERN-OBERTI, JUAN PABLO

Buenos Aires

Congreso; Reunión de las Sociedades de Biociencias 2021; 2021

SAIC-SAI-AAFE-Nanomed Ar

Hyperthyroidism(HP) induces reproductive disorders like preterm birth and lactation failuresand could influence immune cells homeostasis. Therefore, our work aimed toevaluate the role of thyroid hormones in leukocytes in milk and placenta. Tothis end, 12 weeks old Wistar rats were injected with T4 (hyper) and euthanizedon day 19, 20 of gestation (G19 and G20), and day 2 of lactation (L2). Placentaand milk immune cells (CD45+, CD3+, CD11b/c+) were analyzed by flow cytometry,and mRNA hormone receptors and cytokines by qPCR. Histological analysis of themammary gland was performed. We observed that fetuses of hyper group (hyper)weighed more in G19 and G20 (p<0.01 p<0,001) compared to controls /Co). Inplacenta of hyper we showed a decrease in β2 thyroid receptor expression(p<0.05) in G19 with an increase in prolactin receptor expression on G20(p<0.05). Furthermore, in hyper prolactin receptor, progesterone receptor,glucocorticoid receptor, and VEGF expression were higher in G20 vs G19(p<0,05 p<0,01 p<0,05). On G19, the percentage and absolute count ofplacental leukocytes were higher in hyper (p<0.05) vs Co. In Co, on G20, weshowed an increase in leukocyte infiltration compared with G19 (p<0.01), however,we did not observe this in hyper. On lactation, the hyper offspring presentedlower weight on days 1 and 2 (p<0,001). In L2, milk had an increase in thepercentage of CD45+ cells in hyper (p<0,05). In addition, CD3+ cells/ µlincreased respect to the Co while the number of CD11 b/c+ cells/ µl diminished(p<0,05). In the hyper, the alveolar area and mammary adipose tissue werelower while mammary connective tissue was higher than Co. These results suggestthat immunity may accompany alterations in fetal growth and in placentalhormonal receptors expression at the end of pregnancy induced by HP. However,their relationship with preterm birth and early lactation impairment needs tobe addressed. Placenta and milk leukocytes would be impacted by HP.@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073786111 1 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:Calibri;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-TRAD;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:Calibri;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-TRAD;}div.WordSection1{page:WordSection1;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073786111 1 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:Calibri;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-TRAD;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:Calibri;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-TRAD;}div.WordSection1{page:WordSection1;}