Apigenin-7-Glucuronide from Urera aurantiaca Inhibits Tumor Necrosis Factor Alpha and Total Nitrite Release in Lipopolysaccharide-Activated Macrophages

MARRASINI, CARLA; COGOI, LAURA; SULSEN, VALERIA; ANESINI, CLAUDIA

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2020

1741-427X

Urera aurantiaca is an Argentinean medicinal and edible species traditionally used to treat symptoms of inflammation. e aim of this study was to evaluate the anti-inflammatory activity of a methanol extract and its major compound. U. aurantiaca aerial parts were extracted with methanol by maceration. A phytochemical analysis was performed, and the extract?s major component, apigenin-7-glucuronide (A7G), was identified by spectroscopic and HPLC methods. e analysis of the inflammatory mediators nitricoxide(NO)andtumornecrosisfactoralpha(TNF- α ) in lipopolysaccharide- (LPS-) stimulated macrophages were used in the evaluationoftheextractandthemajorcompoundanti-inflammatoryeffects.eextractreducedLPS-augmentedNOreleasefrom 100 μ g/mL (27%), reaching the highest inhibition at 1000 μ g/mL (96.3%), while A7G reduced it 30.7% at 1 μ g/mL, and its maximum effect was 97.1% at 10 μ g/mL.IntheTNF- α model, the extract at 500 and 1000 μ g/mLreducedLPS-augmentedTNF- α by 13.5% and 93.9%, respectively; meanwhile, A7G reduced it by 26.2% and 83.8% at 5 and 10 μ g/mL, respectively. U. aurantiaca popular use was validated. In the present study, for the first time, A7G was isolated from U. aurantiaca ; furthermore, A7G showed anti-inflammatory effect in the macrophage cell line RAW264.7 (ATCC) and seems to be responsible for the extract anti-inflammatory effect.