Identification of Centella Asiatica Extract in a Cosmetic Cream

LÓPEZ PAULA; CATALANO VANINA; CUFRE INGRID; TARCAYA VERÓNICA; COGOI LAURA; BROUSSALIS ADRIANA

International Journal of Phytocosmetics and Natural Ingredients

International Journals of Integrated Sciences

Lugar: Boston; Año: 2015 vol. 2 p. 16 - 18

Regulatory requirements call for the identification of any claimed active ingredient in a cosmetic formulationwhile quantification of its amount in the product is not mandated. Quality control of productsrequires certain analysis. They need to assure dependable results for better identification of ingredientsin cosmetic products as well as raw materials. Active ingredients for Centella cosmetic creaminclude extracts of Centella asiatica. This ingredient is a plant extract that is profiled by chromatographicanalysis. The analysis is also a suitable detection method to determine presence of this activeingredient in cosmetic products. Thin layer chromatographic (TLC) analysis, a traditional method ofprofiling the extract of Centella asiatica, was employed along with the new approach of using High PerformanceLiquid Chromatography (HPLC). The TLC and HPLC methods used for the Centella creamwere developed based on the European Pharmacopoeia monograph for Centella asiatica plant material.A normal phase TLC analysis was performed using Silicagel as a stationary phase and a mobile phasecontaining water. An RP18 column and a linear gradient were used in the HPLC method. This studyreports presence of asiaticoside in samples of Centella cream both by TLC and HPLC. The presence ofmadecassoside was also determined by TLC. This paper reports use of these chromatographic methodsfor the determination of Centella asiatica extract in a cosmetic cream and finds it applicable for qualitycontrol purposes of other cosmetic creams containing Centella asiatica.