APICHELA Silvana Andrea
congresos y reuniones científicas
GLYCOCONJUGATES CHARATERIZATION OF LAMA OVIDUCT BY LECTINHISTOCHEMESTRY
APICHELA, SILVANA A.; JIMÉNEZ-DÍAZ, MARÍA. A.; MICELI, DORA C.
Tafí del valle, Tucumán
Jornada; XXIV JORNADAS CIENTÍFICAS DE LA ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN; 2007
ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN
Oviduct-sperm binding is a reversible process that appers to involve epithelial cell membrane oligosaccharides and sperm surface Ca2+ dependent lectins. The glycoconjugate involved in the llama sperm reservoir formation is still unknown. Llamas are induced ovulators, although ovulation only occurs when, at the time of mating, the dominant follicle is larger than 7 mm. This study characterizes the glycosilate residues in the llama oviduct by using fluorescent lectins. Oviducts were classified into two groups according to the size of the dominant follicle: >7 mm or <7 mm. Uterotubal Junction, Isthmus and Ampulla where separated, fixed (formaldehyde 10% pH 7.2) and embedded in paraffin. Sections were labelled with WGA, WGAs, UEA 1, DBA, RCA 120, Con A, PSA, LCA, PHA E, PHA L, GSL and SJA lectins FITC/rodhamine conjugated and observed by Confocal Laser Scanning Microscopy. The glicocalix along the three oviductal segments showed abundant µ-mannopyranosyl, µ-glucopyranosyl, N-acetyl glucosamine, and N-acetylneuraminic acid residues and few µ-linked N-acetyl galactosamine and b-galactosyl residues. Neither µ-L-fucopyranosyl nor b-N-acetyl galactosamine residues were observed. No differences where found between ovarian stages. The sugar residues found could be related to a sperm reservoir oviductal function.