APICHELA Silvana Andrea
SEGMENT-SPECIFIC EXPRESSION OF MMP/TIMP IN THE OVIDUCT OF LLAMA (LAMA GLAMA) AND GELATINOLYTIC ACTIVITY IN THE OVIDUCTAL FLUID
ZAMPINI, RENATO; APICHELA, SILVANA A.; SARI, LUCIANA; ANGIONO, G; LOMBARDO, D; MICELI DORA C.; ARGAÑARAZ, MARTÍN E
INTERNATIONAL JOURNAL OF MORPHOLOGY
SOCIEDAD CHILENA DE ANATOMÍA
Lugar: TEMUCO; Año: 2017 vol. 35 p. 615 - 623
Oviductal molecules have the potential to improve the reproductive biotechnologies. In camelids, knowledge and assessment of the oviductal environment are necessary to successfully develop species-specific 23reproductive technologies, especially because of the camelids reproductive particularities. Among theoviductal factors, the matrix metalloproteinases/tissue inhibitor of matrix metalloproteinases system 25(MMPs/TIMPs) should be investigated more thoroughly due to their participation in reproductive processes.Consequently, the current study assayed gene and protein expression of MMPs throughout the llama oviduct. MMPs zymogen and active forms in the oviductal fluid were also characterized. MMP2 and MMP9 transcripts were detected in ampulla, isthmus, utero-tubal junction and papilla, being MMP2 and MMP9 2.15 and 1.10 folds higher in papilla than in ampulla, respectively. In addition, differences in immunolocalisation of MMP2 and MMP9 between the epithelial mucosa layers of the oviductal segments were observed. The presence of MMPs in the epithelium suggests their secretion into the oviductal lumen. Coincidently, bands of 62 and 94 kDa, corresponding to MMP2 and MMP9 were detected by zymography in the oviductal fluid. Treatment with an exogenous activator (APMA) suggests that they are present as proMMPs. TIMP2 and TIMP1, the specific inhibitors of MMP2 and MMP9, respectively, were expressed in each oviductal segment, indicating a well-regulated control of MMP proteolytic activity in the oviduct. These findings prove that the llama oviduct produces and secretes MMPs into the oviductal lumen, suggesting that these enzymes may have an unknown role in the preparation of the oviductal environment for gametes, fertilization and early embryo development in camelids.