A 5' RNA element promotes dengue virus RNA synthesis on a circular genome.

FILOMATORI CV; LODEIRO MF; ALVAREZ DE; SAMSA MM; PIETRASANTA L; GAMARNIK AV

GENES & DEVELOPMENT.

Año: 2006 vol. 20 p. 2238 - 2249

0890-9369

The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5´ element named SLA. We demonstrated that RNA-RNA interactions between 5´ and 3´ end sequences of the viral genome enhance dengue virus RNA synthesis only in the presence of an intact SLA. We propose a novel mechanism for minus-strand RNA synthesis in which the viral polymerase binds SLA at the 5´ end of the genome and reaches the site of initiation at the 3´ end via long-range RNA-RNA interactions. These findings provide an explanation for the strict requirement of dengue virus genome cyclization during viral replication.