Does zinc deficiency exacerbate metabolic disorders induced by high-fat diet?

PAEZ DIAMELA TATIANA; GIACOMAZZI, JULIA; CIANCIO NICOLAS; PIOVAROLI FLORENCIA; MAGALDI ROCIO; SOSA, AGUSTINA; MARTÍNEZ TAMBELLA, JOAQUÍN; SORIA, DAMIÁN A.; RAMIREZ, HERNÁN; SARAVIA MELISA; ZAGO, VALERIA; SCHREIER, LAURA; MENDES GARRIDO FACUNDO; ELESGARAY, ROSANA; CANIFFI, CAROLINA; ARRANZ CRISTINA; TOMAT ANALIA LORENA

CABA

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020. LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC) LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS); 2020

Introduction: Moderate zinc deficiency during fetal and postna tal life is associated with cardiovascular and metabolic disorders in male adult rats. Objective: We evaluated if fetal and postnatal zinc deficiency exacerbates the extent of adiposity and metabolic dysfunction induced by high fat diet (HFD) in male adult rats. Meth ods: Female Wistar rats received low(L:8ppm) or control(C:30ppm) zinc diets from pregnancy to offspring weaning. C male offspring continued with C(C) or HFD (60% of total calories)(CH) diets. L off spring were fed L (L) or L and HFD(LH) diets. At day 81, plasma adiponectin levels, oral glucose tolerance test (OGTT)and mor phology and oxidative state of retroperitoneal adipose tissue (RAT) were measured. Results: CH and LH had higher body weight (C:418±13;CH:505±9*;L:401±10;LH:444±5Ɨǂg) and showed an in crease of RAT weight, a decrease of adipose cells density and adi pocytes hypertrophy (C:4958±388;CH:9621±586*;L:8130±448*; LH:11833±440Ɨǂμm2 ). LH showed lower body weight and higher adipocyte area than CH. HFD induced a decrease of plasma ad iponectin levels(C:8.3±0.6; CH:6.5±0.4*;L:8.6±0.9; LH:6.4±0.1Ɨμg/ ml) and functional changes in RAT like a decrease of SOD and catalase antioxidant activities and an increase of TBARS (C:0.21 ±0.02;CH:0.38±0.04*;L:0.40±0.06*;LH:0.43±0.05*pmol MDA/mg prot).L rats showed an increase in oxidative stress in RAT. LH and CH showed an increase of OGTT curve area(C:27797±504;CH:3 0827±971*;L:27826±809; Lh:34851±1344Ɨǂmin.mg/d). L, LH and CH showed higher plasma glucose levels after 3 hours of glucose overload. Zinc deficiency exacerbated alterations induced by HDF. Two way ANOVA, Bonferroni post-test,mean±SEM,*p