INVESTIGADORES
TOMAT Analia Lorena
artículos
Título:
Renal and vascular nitric oxide system in reduced renal mass saline hypertension
Autor/es:
ARRANZ C; TOMAT A; FELLET A; GARCIA J; BALASZCZUK AM; COSTA MA
Revista:
NEPHRON
Editorial:
Karger
Referencias:
Lugar: Londres; Año: 2003 vol. 95 p. 36 - 42
ISSN:
0028-2766
Resumen:
Background: Reduction in renal mass is associated with
several structural and functional adaptations including
compensatory renal growth and hemodynamic changes.
The mediators of the renal hemodynamic adaptations
have not been definitively identified. Several investigators
have postulated that nitric oxide (NO) is involved
this physiological mechanisms. The purpose of this
study was to evaluate the role of vascular and renal NO
pathway in the model of subtotal nephrectomy-salt load
hypertension. Materials and Methods: Wistar rats with
75% renal mass reduction (RMR) and saline load were
studied during 4 weeks. Weekly, indirect systolic blood
pressure (SBP) were measured. One week after nephrectomy,
animals were divided in two groups, hypertensive
(SBP 1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
75% renal mass reduction (RMR) and saline load were
studied during 4 weeks. Weekly, indirect systolic blood
pressure (SBP) were measured. One week after nephrectomy,
animals were divided in two groups, hypertensive
(SBP 1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
several structural and functional adaptations including
compensatory renal growth and hemodynamic changes.
The mediators of the renal hemodynamic adaptations
have not been definitively identified. Several investigators
have postulated that nitric oxide (NO) is involved
this physiological mechanisms. The purpose of this
study was to evaluate the role of vascular and renal NO
pathway in the model of subtotal nephrectomy-salt load
hypertension. Materials and Methods: Wistar rats with
75% renal mass reduction (RMR) and saline load were
studied during 4 weeks. Weekly, indirect systolic blood
pressure (SBP) were measured. One week after nephrectomy,
animals were divided in two groups, hypertensive
(SBP 1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
75% renal mass reduction (RMR) and saline load were
studied during 4 weeks. Weekly, indirect systolic blood
pressure (SBP) were measured. One week after nephrectomy,
animals were divided in two groups, hypertensive
(SBP 1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Reduction in renal mass is associated with
several structural and functional adaptations including
compensatory renal growth and hemodynamic changes.
The mediators of the renal hemodynamic adaptations
have not been definitively identified. Several investigators
have postulated that nitric oxide (NO) is involved
this physiological mechanisms. The purpose of this
study was to evaluate the role of vascular and renal NO
pathway in the model of subtotal nephrectomy-salt load
hypertension. Materials and Methods: Wistar rats with
75% renal mass reduction (RMR) and saline load were
studied during 4 weeks. Weekly, indirect systolic blood
pressure (SBP) were measured. One week after nephrectomy,
animals were divided in two groups, hypertensive
(SBP 1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
75% renal mass reduction (RMR) and saline load were
studied during 4 weeks. Weekly, indirect systolic blood
pressure (SBP) were measured. One week after nephrectomy,
animals were divided in two groups, hypertensive
(SBP 1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Materials and Methods: Wistar rats with
75% renal mass reduction (RMR) and saline load were
studied during 4 weeks. Weekly, indirect systolic blood
pressure (SBP) were measured. One week after nephrectomy,
animals were divided in two groups, hypertensive
(SBP 1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
1 140 mm Hg) and normotensive (SBP ! 140 mm
Hg). Urinary excretion of nitrates and nitrites (NOx), urinary
chemioluminiscence levels and NOS activity in the
left kidney and in the thoracic aorta artery were determined
at the fourth week after subtotal nephrectomy.
Results: Urinary excretion of sodium was higher in normotensiveUrinary excretion of sodium was higher in normotensive
rats than hypertensive rats and in both groups
this parameter was higher than in sham rats. NOx excretion
and NOS activity in the different nephron segments
were higher in normotensive rats than in the hypertensive
ones. In contrast, NOS activity in aorta sections and urinary
chemiluminescence levels in hypertensive animals
were enhanced compared with normotensive rats. These
parameters were higher in both groups of nephrectomized
rats than in sham ones. Conclusion: This study provides
evidence to support the fact that the activation of the
renal NO system is an important mechanism whereby the
remnant kidney regulates sodium and water balance,
contributing to control the arterial blood pressure in the
renal mass reduction and saline load model.
evidence to support the fact that the activation of the
renal NO system is an important mechanism whereby the
remnant kidney regulates sodium and water balance,
contributing to control the arterial blood pressure in the
renal mass reduction and saline load model.
Conclusion: This study provides
evidence to support the fact that the activation of the
renal NO system is an important mechanism whereby the
remnant kidney regulates sodium and water balance,
contributing to control the arterial blood pressure in the
renal mass reduction and saline load model.
Copyright © 2003 S. Karger AG, Basel