Organ-specific changes in the expression of mannose-6-phosphate receptors during postnatal development in rats

JOFRE G; BALMACEDA V; SARTOR T; CARVELLI L; BARRERA P; SOSA MA

CELLS TISSUES ORGANS

KARGER

Año: 2008 vol. 190 p. 27 - 33

1422-6405

The significance of the coexistence of 2 mannose-6-phosphate receptors (MPRs) in most cell types still remains poorlyunderstood. In this study, expression of the cation-dependent MPR (CD-MPR) and the cation-independent MPR(CI-MPR) was measured by Western blot in rat organs at 3 ages, i.e. in newborn and 10- and 90-day-old rats. It was observed that expression of the CI-MPR tends to diminish from newborns to adults in 5 of the 6 organs studied, whereas the CD-MPR did not show a clear tendency over time. In pancreas, conversely, both MPRs increased progressively fromnewborns to adults. The activity of 2 acid hydrolases was also measured at the different ages, and a low correlation was found with the expression of the 2 MPRs. With the exception of the pancreas, it is possible that the CI-MPR is mostly occupied with the clearance of insulin-like growth factor-II at early stages of development, and that later both MPRs may participate in the maturation of the lysosomal apparatus. We propose that in the pancreas, both receptors may be involved in increasing the proteolytic activity of this exocrine gland during postnatal development.