ROLE OF THE CAVEOLAE IN THE REMODELING OF MATERNAL SPIRAL ARTERIES DURING THE FORMATION OF THE PLACENTA.

REPPETTI J; SEHAYIANE A; RECA A; BELTRAMONE N; PARBORELL F; DAMIANO A; MARTÍNEZ N.

Buenos Aires

Congreso; REUNION CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Extravillous trophoblast (EVTs) cells of the human placenta migrate and invade theuterine decidua and adopt an endothelial phenotype to modify utero-placental arteries.These events also require a successful cooperation between EVTs and endothelial cells(ECs).Caveolae are membrane domains that compartmentalize intracellular signalingpathways to orchestrate different cellular events, as cell migration and invasion.However, the importance of caveolae during placentation remains unknown.Our aim was to evaluate the role of caveolae in EVTs migration, endovasculardifferentiation and interaction with ECs.Swan 71 cell line was treated with 5 mM methyl-beta-cyclodextrin (MCD) to disruptcaveolae. Conditioned medium (CM) was collected. Cav-1 expression was tested byWestern blot and immunofluorescence. Migration was assessed by wound healingassay. Endovascular differentiation was evaluated by formation of tube-like structures inplates coated with Matrigel.To evaluate the interaction between EVTs and ECs (EA.hy926), proliferation (evaluatedby MTT assay), migration and tube formation assays were performed in the presence ofCM obtained from Swan 71 cells.Disruption of caveolae significantly decreased Cav-1 expression (n=5; p