Hyperosmolar stress affects TRPV-1 expression and the physiological functions of human trophoblast

J. REPPETTI ; A. SEHAYIAN; N. SZPILBARG; M. FARINA; A.E. DAMIANO; N. MARTÍNEZ,

Simposio; Latin American Society for Maternal Fetal Interaction and Placenta; 2017

Hyperosmolar stress may be an important stressor that alters normaldevelopment of embryos or placentation. Transient receptor potentialvanilloid 1 (TRPV-1) is activated by hyperosmolarity and participates inmany cellular processes such as apoptosis, authophagy, and others.Objective: To evaluate the effect of hyperosmolar stress on cell viability,migration and invasion, and the contribution of TRPV-1 to these processesin first trimester human trophoblast cells.Methods: Swan-71 cell line (human trophoblastic cells) was cultured incomplete DMEM-F12 and sucrose hyperosmolar solution was added for24 h with or without capsaicin (CPZ, 1 mM) and capsazepine (CPZ, 10mM). TRPV-1 protein expression was analysed by Western blot. Cellviability was analysed by MTT, LDH and b-hCG assays. Apoptosis wasevaluated by Bax expression, DNA fragmentation and TUNEL assay, andauthophagy by monodansylcadaverine (MDC) assay. Migration wasassessed by wound healing assay, activity of metalloproteinases (MMPs)by zymography, and invasion was evaluated in transwells pre-coatedwith Matrigel.Results: In hyperosmolar conditions, TRPV-1 expression was increased(1.3 ± 0.1 fold) (P