INVESTIGADORES
MARTINEZ Nora Alicia
congresos y reuniones científicas
Título:
Caveolin-1 in human preeclamptic placentas.
Autor/es:
CASTRO PARODI M; ABAN C; MARTÍNEZ N,; HERLAX V; MATE S; FARINA M; DAMIANO A
Lugar:
Mar del Plata
Reunión:
Simposio; VI LatinAmerican Symposium on Maternal-Fetal Interaction & Placenta; 2015
Resumen:
Caveolin-1 is a
raft-associated protein involved in many cellular events as
cell
differentiation. It is thought that caveolin-1 may play an important role
in the negative
regulation of the cell cycle, retarding mitosis to halt proliferation.
Emerging data
establish that disturbances in trophoblast differentiation
are related to
pathological conditions such as preeclampsia.
Unlike the
situation reported in other differentiated cells, a normal syncytialization
process has been
associated with a reduction in the number of
caveolae, and a
decreased expression of caveolin-1. It is known that lipid
rafts are membrane
domains rich in sphingolipids and cholesterol. In
preeclamptic
placentas, we have previously found an increase in sphingomyelin
in the apical
membrane of syncytiotrophoblast without changes
in cholesterol
amount. However, the expression of caveolin-1 has not been
investigated yet.
Objective:
To study the expression and localization of caveolin-1 in preeclamptic
placentas.
Methods:
Caveolin-1 expression in normal (n¼8) and preeclamptic (n¼8)
placentas was
determined by RT-PCR, Western Blot and
immunohistochemistry.
Detergent-resistant
Membranes (DRMs) were prepared from apical and
basal vesicles from
normal and preeclamptic placentas by sucrose gradient
centrifugation and
caveolin-1 protein expression was detected in each
fraction.
Results:
At transcriptional levels, RT-PCR experiments showed no differences
between normal and
preeclamptic placentas. In normal placentas,
caveolin-1
localized in the apical membrane of the syncytiotrophoblast
and in the
endothelium. However, in preeclamptic placentas, caveolin-1
was almost undetectable. Accordingly with this result, Western
blots
showed a significant
decrease of caveolin-1 protein in the apical DRMs
from preeclamptic
placentas compared to normal ones.
Conclusions:
Our findings
suggest that caveolin-1 was almost undetectable
in preeclamptic
placentas. Although in normal placenta, the reduction
in caveolin-1
throughout pregnancy may simply be a feature of the loss of
lateral cell
membranes following cell fusion, in preeclamptic placentas the
marked decreased of
this protein may be associated with failures in the
syncytialization process.