Lipase activity from Aspergillus niger MYA 135 on agarized medium

. ROMERO C, RODRÍGUEZ E. PERA L, BAIGORÍ M.

Tafi del Valle. Tucuman

Jornada; XXII Jornadas Cientificas Asociación de Biología de Tucumán Tafi del Valle Tucumán; 2005

Sociedad de Biologìa

LIPASE ACTIVITY FROM ASPERGILLUS niger MYA 135 ON AGARIZED MEDIUM Romero C, Rodr.guez E, Pera L, Baigor. M. PROIMI Av. Belgrano y Pje. Caseros, 4000 Tucum.n. Tel: 4344888, E-mail: baigori@hotmail.com Introduction. Lipases (E 3.1.1.3) are biotechnologically important enzymes that catalyze the hydrolysis and synthesis of a wide array of esters. Industrial demand for new sources of lipases is continuing to stimulate the isolation and screening of new lipolytic microorganisms. Objective. Evaluation of different agarized media for selection of fungal producer lipases. Materials and methods. Hyphae of Aspergillus niger (ATCC MYA 135) were grown at 30°C on MB agar plus rhodamine B medium supplemented with either olive oil or different kind of Tween (20, 40, 60, and 80). Both MB agar medium and MB agar medium supplemented with olive oil were also evaluated. MB (g/l): sacarose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06; pH7. Lipase production was monitored by irradiating plates with UV light at 350 nm. Results and conclusions. The presence of rhodamine B and either Tween 40 or olive oil showed the same sensibility for lipase production. A little bit lower sensibility in medium supplemented with only olive oil was observed. Interesting, the presence of Tween 40 also delay conidiation process. This work was supported by grant CABBIO 2000 cod 012.