INVESTIGADORES
ROJAS Natalia Lorena
congresos y reuniones científicas
Título:
INFLUENCE OF THE CARBON AND NITROGEN SOURCES ON INULINASE PRODUCTION BY ASPERGILLUS KAWACHII IN SUBMERGED CULTURES
Autor/es:
CHESINI, M.; CAVELLO, I. A; N.L. ROJAS; CAVALITTO, S. F.
Reunión:
Encuentro; VIII Encuentro Latinoamericano y del Caribe de Biotecnología REDBIO-Argentina 2013; 2013
Resumen:
Inulinase is an industrial enzyme which has caught the attention of researchers.
Inulinases are used for production of high fructose syrups, fructooligosaccharides and
ethanol used in pharmaceutical and food industry. Inulin -a carbohydrate reserve
composed of D-fructose- is widely prevalent in many plants, particularly in roots, and
tubers such as dahlia, chicory and yacon. The production of inulinase has been
reported from several fungal, yeast and bacterial strains. The aim of this work is to
investigate the optimum conditions for the maximum production of inulinase by
A.
kawachii
. In a previous work we reported that yacon extract was the best carbon
source for the production of
A. kawachii
inulinase. Here, the effect of glucose, fructose,
sucrose, pure inulin, sugarcane molasses and soybean molasses in combination with
yacon extract at a concentration of 5g/l were investigated. It was possible to increase 2
fold enzyme activity when yacon was used in combination with soybean molasses.
Glucose, fructose and sucrose inhibit inulinase production. The influence of various
nitrogen sources (yeast extract, urea, ammonium nitrate and sodium nitrate) on the
inulinase production was studied. Yeast extract was found to be most suitable one
increasing 1.75 fold enzyme activity whereas ammonium ion was inhibitory.