A bacterial protease inhibitor delivered subcutaneusly limits APCs lysosomal proteolysis and increases immunogenicity of co-administered Ag

CORIA, LORENA; IBAÑEZ, ANDRÉS; CARABAJAL, MARIANELA; BERGUER, PAULA; BRUNO, LAURA; RISSO, GABRIELA; GONZALEZ COBIELLO, PAULA; FRANK, MARIA FERNANDA; PASQUEVICH, KARINA; CASSATARO, JULIANA

Seattle

Simposio; Keystone Symposia on Molecular and Cellular Biology: The modes of action of Vaccine adjuvant; 2014

Keystone Symposia

Our previous results demonstrated that a Brucella spp. protein (Bp) is a broad spectrum protease inhibitor as it can inhibit proteases present in the stomach and gut (mostly serine proteases). In this work we demonstrated that Bp also possesses inhibitory activity on lysosomal proteases (cysteine proteases). Bp partially reduced the proteolytic activity of commercial cysteine proteases in vitro (P