Complete sequence of a blaNDM-1-harboring plasmid in an Acinetobacter bereziniae clinical strain isolated in Argentina

BROVEDAN M; MARCHIARO P; MORAN BARRIO J; CAMERANESI MM; CERA G; RINAUDO M; VIALE AM; LIMANSKY AS

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

AMER SOC MICROBIOLOGY

Lugar: Washington; Año: 2015

0066-4804

We describe here the first case of an NDM-1-producing Acinetobacter bereziniae isolate in Argentina, called HPC229. Analysis of whole-genome sequencing performed with pyrosequencing (454 Genome Sequencer FLX system; Roche Diagnostics) demonstrated that the blaNDM-1 metallo-b-lactamase gene was located in HPC229 in a 44,560-bp plasmid, designated pNDM229.To our knowledge, only one other A. bereziniae NDM-1-producing clinical isolate has been recently reported (CHI-40-1), and the blaNDM-1 gene was located in this strain in a conjugative plasmid. However, a number of differences were noted between HPC229 and CHI-40-1 and between the plasmids carried by them. Thus, Acinetobacter species other than A. baumannii can act as reservoirs of blaNDM genes and most probably contribute to their spreading among clinically relevant Enterobacteriaceae species.