INVESTIGADORES
FAVALE Nicolas Octavio
congresos y reuniones científicas
Título:
PARTICIPATION OF GLYCOSPHINGOLIPID BIOSYNTHESIS IN RENAL COLLECTING DUCT CELL DIFFERENTIATION
Autor/es:
PESCIO L; LEOCATA NIETO F; FAVALE N; FERNANDEZ-TOMÉ MC; STERIN-SPEZIALE N
Lugar:
Mar del Plata , Buenos Aires, Argentina
Reunión:
Congreso; XLIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; 2007
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
MDCK
is a renal collecting duct cellular line that conserves the capacity to
differentiate in particular conditions of cell culture. To differentiate, they
acquire a polarized phenotype characterized by the presence of apical and
basolateral membrane domains with different protein and lipid composition.
Glycosphingolipids (GSL) predominate in the apical membrane. It has been
previously reported that extracellular hypertonicity induces cell
differentiation, but the mechanism involved is not well understood. In this
study, we have examined the role of GSL biosynthesis in collecting duct
celldifferentiation. Confluent MDCK cells were submitted to 300 mM NaCl for 24
and 48h. Glycosphingolipid metabolism was determined by using C-galactose as radioactive
precursor and cell differentiation was followed by using fluorescent anti
gp135, a marker of apical membrane, and visualized by confocal fluorescent microscopy.
The results demonstrate that hypertonicity induced an early increase in GSL
biosynthesis and the formation of apical membrane domain reflected by the
apical accumulation of gp135. The pretreatment with PDMP, an inhibitor of
Glucosylceramide synthase, evoked a 70% decrease in GSL biosynthesis and
induced the disappearing of gp135 accumulation in apical membrana domain. From
these results we conclude that GSLbiosynthesis play a central role inMDCKcells
differentiation.