INVESTIGADORES
FAVALE Nicolas Octavio
congresos y reuniones científicas
Título:
Intranuclear Speckle Localization Of Cytidylyltransferase Alpha Serves As A Regulatory Mechanism
Autor/es:
FAVALE NO; SANTACREU B; GERARDI G; STERIN-SPEZIALE NB
Lugar:
Puerto Madryn, Chubut, Argentina
Reunión:
Congreso; XLVI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; 2010
Institución organizadora:
SAIB
Resumen:
CCTa
is an amphitropic enzyme, but in most cell lines it has been localized
predominantly in the nucleus. We have demonstrated that hypertonicity (stimulus that evokes
differentiation) induces lamin A/C distribution to nucleoplasmic speckles and CCTa colocalizes with these intranuclear
structures. In the present work we evaluated the
importance of CCTa
intranuclear redistribution and its relationship with enzyme activity and
PtdCho synthesis. Silencing of Lamin A/C caused decrease in CCTa
speckles number and an increase in CCTa activity. To evaluate CCTa
speckle dynamics, cells were treated with oleic acid. Treatment was able to mobilize
CCTa
outside of speckles and was redistributed to nuclear envelope. Interestedly CCTa
relocalization occurred only in MDCK cells subjected to hypertonicity but not in
control cells. Oleic acid stimulus induced higher increase in PtdCho synthesis
in control cells than hypertonic treated cells. No changes were observed in CCTa
activity. Results herein demonstrate that oleic acid induces CCTa
redistribution which occurs only in hypertonicity subjected MDCK cell, suggesting
that in differentiated cells, CCTa-speckles
are necessary for its re-localization. Under hypertonic condition intranuclear
CCTa
distribution in nuclear speckles could serve as a localized platform that
facilitates CCTa
mobilization.