Genomic studies of microorganisms in phytoremediation of contaminated soils with hydrocarbons under Populus sp. Culture

BADARIOTTI, E.H.; YANG, P.; CANULLO, R.; SORIA, N.; SAYAGO, P.; MOLINA, M.G.; JUNCOSA, F.

Foz do Iguaçu

Workshop; IV Workshop Latino-Americano de PGPR.; 2018

The contamination of soils by hydrocarbons has a marked effect on the modification of their properties and the development of microorganisms. Furthermore, the persistence of these components in soils is prolonged over time. In recent years, the use of biological methods in the remediation of contaminated sites has increased. The implementation of the phytoremediation technique implies the use of plants and their associated microorganisms at the rhizosphere level, capable of tolerating and detoxifying persistent contaminants in the soil.Certain groups of bacteria under certain conditions are capable of intervening in the degradation of hydrocarbons and have the metabolic capacity to convert these agents into CO2 and H2O. Microorganisms that use hydrocarbons as a carbon source must have enzymes systems such as the oxygenases group, capable to produce metabolites that lead to hydrocarbons degradation. Gordonia sp. possesses the ability to tolerate and remove compounds containing hydrocarbons, aromatic, and aliphatic.The objective of this work was to carry out a genomic analysis study of a bacterial strain of the genus Gordonia sp, isolated and identified from contaminated soil with diesel fuel. The identification was made by a high performance sequencing system to obtain the complete genome. Gordonia sp was associated with Populus deltoides, in agricultural land. The concentration of the contaminant was 10,000 mg. kg -1ss.On the basis of the results of the complete obtained sequence was inferred that the bacteria has the capacity to produce enzymes such as aldehyde and alcohol dehydrogenases, monooxygenases and hydroxylases; all able to metabolize different hydrocarbons using different metabolic pathways. Among those identified, Alcohol dehydrogenase (EC 1.1.1.1), with an identity range between 69 to 91%; Salicilate hydroxylase (EC 1.14.13.1), with a range between 66 to 81% and the AlkaneSulfonatemonooxygenase (EC 1.14.14.5), with an identity range between 68 to 87% respect to those corresponding to other microorganisms cited by the GenBank.These results of genomic sequencing, would indicate the bacterial potentiality in the enzymes production which could be actively involved in compounds degradation presents in the hydrocarbons mix as is the case of diesel fuel.