Evaluation of growth promoting activity of two strains identified as Bacillus amyloliquefaciens in tomato culture

ALBARRACÍN ORIO, ANDREA G.; P. SAYAGO; DANIEL A. DUCASSE; F. JUNCOSA

Foz do Iguaçu

Workshop; IV Workshop Latino-Americano de PGPR; 2018

Evaluation of growth promoting activity of two strains identified as Bacillus amyloliquefaciens in tomato cultureSayago, P1*; Juncosa, F1; Albarracín Orio, A1; Ducasse D2.1 UE ?IRNASUS? Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sánchez Labrador S.J. (CONICET ? UCC), Córdoba, Argentina. 2 Instituto de Patología Vegetal-CIAP-INTA, Córdoba, Argentina. pamesayago@gmail.comPlant growth promoting rhizobacteria (PGPR) are currently viewed as a sustainable alternative to achieve increases in crop production. The term PGPR includes a group of free living bacteria, capable of colonizing the plant ́srhizosphere, resulting in positive effects on their growth and development. The mechanisms through which they interact positively with plants are diverse, and they include the production of growth regulators such as abscisic acid (ABA), gibberellins (GAs), indole acetic acid (IAA); nitrogen fixation, and solubilisation of nutrients, among others. The objective of this work was to evaluate the growth promotion capacity of two strains of Bacillus amyloliquefaciens, in tomato plants. These strains, designed in this work asBa1 and Ba2, were selected according to biochemical characteristics that would indicate a possible positive effect on plants.For the purposes of this work, Moneymaker tomato cultivar was selected, and an essay was carried out under greenhouse conditions. Tomato seeds were previously subjected to a bio-priming process, inoculating them with Ba1 and Ba2 for 24 h at 28 ± °C in BHI (Brain Heart Infusion) culture medium. As a control, seeds were soaked in equal medium and conditions but without bacteria. After 60 days from sowing, plantlets were subjected to a second inoculation. At 80 days post-emergence, moment in which the crop was fructifying; length of roots, length of the aerial part and dry weight were evaluated. Strain Ba2 showed a clear plant growth promoting activity, manifestingstatisticaly significant differences between the treatments in the variables dry weight and length of the aerial part.Keywords: growth promotion; tomato; PGPR.Financial Support: UE ?IRNASUS? UCC ? CONICET. INTA.