Sequence variation of Epstein-Barr virus: viral types, geography, codon usage and diseases

CORREIA SAMANTHA; VENTURINI CRISTINA ; COHEN JEFFREY I; WHITE ROBERT E; FARRELL PAUL J; WEGNER FANNY; MIDDELDORP JAAP M; BASSANO IRENE; BREUER JUDITH ; BRIDGES RAY; PALSER ANNE; LORENZETTI MARIO ALEJANDRO; KELLAM PAUL

JOURNAL OF VIROLOGY

AMER SOC MICROBIOLOGY

Lugar: Washington; Año: 2018

0022-538X

138 new Epstein-Barr virus (EBV) genome sequences have been determined. 125 of these and 116 24 from previous reports were combined to produce a multiple sequence alignment of 241 EBV 25 genomes, which we have used to analyze variation within the viral genome. The type 1/type2 26 classification of EBV remains the major form of variation and is defined mostly by EBNA2 and EBNA3, 27 but the type 2 SNPs at the EBNA3 locus extend into the adjacent gp350 and gp42 genes, whose 28 products mediate infection of B cells by EBV. A small insertion within the BART miRNA region of the 29 genome was present in 21 EBV strains. EBV from saliva of USA patients with chronic active EBV 30 infection aligned with the wild type EBV genome, with no evidence of WZhet rearrangements. The 31 V3 polymorphism in the Zp promoter for BZLF1 was found to be frequent in nasopharyngeal 32 carcinoma cases both from Hong Kong and Indonesia. Codon usage was found to differ between 33 latent and lytic cycle EBV genes and the main forms of variation of the EBNA1 protein have been 34 identified.