NONYLPHENOL INDUCES CYTOSKELETAL CHANGES AND RELEASE OF PROINFLAMMATORY MEDIATORS IN RAT SERTOLI CELLS IN VITRO

VALLES, ANA SOFIA; GODOY SEPÚLVEDA RODRIGO; ORESTI GERARDO MARTÍN; MORENO RICARDO D; AVELDAÑO MARTA; REYES JUAN

BUENOS AIRES

Congreso; SAIB; 2017

SAIB

NONYLPHENOL INDUCES CYTOSKELETAL CHANGES AND RELEASE OF PROINFLAMMATORY MEDIATORS IN RAT SERTOLI CELLS IN VITROAna Sofia Vallés (1), Rodrigo Godoy Sepúlveda (2), Gerardo Martín Oresti (1), Ricardo D. Moreno (3), Marta Isabel Aveldaño (1), Juan G Reyes (2)(1) Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Bahía Blanca, Argentina. (2) Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile. (3) Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile.Nonylphenol (NP), an alkylphenol present in plasticizers, is an endocrinedisrupting chemical that is potentially dangerous for male reproduction in mammals including man. Because Sertoli cells (SC) provide structural and metabolic support to germ cells, in this study the hypothesis that exposures of SC to NP affect their metabolic functions and the production of bioactive molecules was evaluated. Primary cultures of SC were preincubated with [3H]arachidonic acid (AA) to label their lipids and then were treated with NP. The NP exposures resulted in increased concentrations of free AA in cells and medium, indicating that such AA was released from [3H]-labeled lipids (p