Actividad antimicrobiana de Microcina J25 en cultivo de macrófagos y determinación de parámetros farmacocinéticos

LÓPEZ, FE; ZENOFF, AM; VINCENT, PA; SALOMÓN, RA; FARÍAS, RN

Rosario - Argentina

Congreso; SAIB 2006; 2006

Sociedad Argentina de Investigaciones Bioquimicas y Biología Molecular

  Microcin J25 (MccJ25) is a low-molecular-mass (2,107 Da) peptide antibiotic, active against strains of the genera Escherichia, Shigella and Salmonella. One of the purposes of the present study was to assess its antibacterial efficacy against the intracellular pathogen Salmonella enterica serovar Newport. To this end, macrophages were collected from BALB/c mice by peritoneal lavage, infected in vitro with S. Newport, exposed to gentamicin and washed. Macrophages were then treated with different concentrations of MccJ25 and counts of viable bacteria were made after the desired incubation period. Results were compared to a control without treatment. Intracellular bacteria were killed by MccJ25. Coincidentally, this demonstrated that the antibiotic is able to traverse the cytoplasmic membrane of eukariotic cells. We also studied pharmacokinetic parameters for MccJ25 in plasma from BALB/c mice, such as area under the concentration-time curve (AUC), half-life (t1/2), clearance (Cl), and volume of distribution (Vd). These values allowed a characterization of both the kinetic of absorption and elimination of MccJ25. Our results could be useful for a potential therapeutic application of this antibiotic